International Journal of Fisheries and Aquatic Studies

Volume 2, Issue 4, 2015


Expression analysis of IL-10 and IFN-γ genes in head kidney of Catla catla (Ham.) fed with
Limonia acidissima L


Author(s): Srinivasan Ponnuraj, Manimekalai Kanagarajan, Dineshbabu Jaganathan, Teepica Priya Darsini Deivamarudachalam

Abstract: Immunostimulants are the most important elements for the control of fish diseases in fresh water aquaculture. Study on the functional mechanism of immunostimulants in fish is vital for elucidating their medicinal property. The effect of dietary supplementation of Limonia acidissima on the immune gene expression of Catla catla was studied. Catla (5 ± 5.5 g) were fed with experimental diets containing 1.5 (T1), 3 (T2) and 6% (T3) Limonia fruit (LF) powder and control diet (T0) for different experimental feeding periods 30, 60, 90, and 120 days. Following each feeding period fish were immunized with heat-killed Aeromonas hydrophila. Tissue samples were collected from kidney after 10 days of immunization. Five fish were sacrificed for tissue collection and gene expression analysis was performed for innate immune genes such as IL-10 and IFN-γ with β- actin as control. There were up-regulations of IL-10 and IFN-γ gene expressions in kidney compared to control. Significantly (p ≤ 0.05) higher expressions of IL-10 and IFN- γ were observed in T2 diet fed fish compared during 30, 60, and 90 days feeding period compared to other groups. This study revealed tissue specific gene expression pattern in catla kidney.

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