Carbamate insecticides, such as carbofuran, are often used in agricultural activities, which contributes to their enduring existence as aquatic contaminants. By measuring the induction of chromosomal abnormalities in the freshwater fish Channa punctatus (Bloch), this study examines the genotoxic potential of carbofuran, a very toxic carbamate pesticide, Channa punctatus is a great bioindicator species. A chromosomal aberration test was performed on Channa punctatus kidney cells to investigate the genotoxic effects of synthetic Carbofuran. In the present investigation C. punctatus were exposed to three sub-lethal concentrations (0.09 mg/L, 0.18 mg/L and 1.45 mg/L) for 96 hours revealed various chromosomal abnormalities. These results validate the clastogenic and genotoxic properties of carbofuran in this aquatic creature, suggesting possible long-term impacts on the genetic integrity, growth and general population health of exposed fish, which also puts human consumers at risk through the food chain.