Author(s): Abdullahi Alhassan, Ogunsakin Oluwanisola Yetunde, Juana Koroma, Abdulkadir Abubakar Dzukogi, Jimkuta Chindo and Adeleye Olubode Thomas

Abstract: This study is aimed at investigating the Trypanosoma potential of stem-back methanolic extract of Ficus sycomorous against Trypanosoma congolense. This investigation is aimed at establishing a study base for subsequent screening of the plant against aquatic trypanosomes. Firstly, the fresh plant of Ficus sycomorous was obtained from Sokoto central market, which was properly identified at Department of Biological sciences, Botany unit, processes and extracted with methanol at Biochemistry department, Usmanu Danfodiyo University Sokoto, Nigeria, the experimental parasite was obtained from department of biochemistry Ahmadu Bellow University Zaria, Nigeria, and the study was conducted at the department laboratory of veterinary helminthology, faculty of veterinary medicine, Ahmadu Bellow University Zaria, Nigeria. Assessment of invitro Trypanosoma activity was performed in triplicate in 96 well microtiter plates (Flow laboratory inc., Mclean Virginia 22101, USA). The concentration of the extracts used are 1mg/ml, 2mg/ml, 4mg/ml, 8mg/ml and 16mg/ml against 20µl of blood containing about 20-23 parasites per field, each concentration of the extract was incubated for 60 minute and the mobile parasite after every 10 minutes of incubation was recorded in mean±SD. To ensure that the effects monitored was that of the extract alone, a set of control was included which contained the parasite suspended in 10% DMSO only as negative control and diminazen aceturate as the positive control. The result shows that the stem-back methanolic extract of Ficus sycomorus dramatically inhibit the parasite growth at all concentration, the number of mobile parasites decreases as the concentration of the extract increases with respect to time. It was found out that, the extract at these concentrations shows No significant potentiality when compare with the positive control (Diminazen aceturate). Furthermore, the lowest concentration that can kill half of the parasite was further extrapolated graphically as IC₅₀ = 561.3 µl/ml. The research therefore suggests that, this plant could be a promising anti-trypanosome which demand for further trials like in-vivo evaluations, molecular investigations, toxicology study, isolation and characterization of the active components.

DOI: 10.22271/fish.2025.v13.i5c.3166

Pages: 235-240  |  116 Views  53 Downloads

Download Full Article: Click Here