Author(s): Shaik Haleema Banafsha, and Motireddy Srinivasulu Reddy

Abstract: The present study evaluated the immunomodulatory and antioxidative responses of Litopenaeus vannamei fed with diets supplemented with probiotics, prebiotics (mannan-oligosaccharides [MOS], fructo-oligosaccharides [FOS], and xylo-oligosaccharides [XOS]), and their combinations. Twelve experimental treatments were formulated, including a control (T1), individual and combined prebiotics, probiotics, and synbiotics. Key immune parameters such as Total Haemocyte Count (THC), Phagocytic Activity (PA), Melanin Production, and levels of immunoglobulin-like proteins were measured alongside antioxidant markers including Superoxide Dismutase (SOD), Catalase (CAT), and Total Antioxidant Capacity (TAC). Results revealed significant enhancements (p < 0.05) in all immunological and antioxidative indices across the treatment groups compared to the control, with the highest values consistently observed in the T12 group (MOS + FOS + XOS + Probiotic). This indicates a synergistic effect between the prebiotic components and probiotic strains (Bacillus licheniformis and Lactobacillus rhamnosus), leading to improved immune defense and oxidative stress resilience in shrimp. The findings suggest that synbiotic-based functional feeds can be a promising strategy for boosting shrimp health and immunity in intensive aquaculture systems. These natural feed additives offer a sustainable alternative to conventional chemotherapeutics, potentially improving disease resistance and production outcomes in commercial shrimp farming.

DOI: 10.22271/fish.2025.v13.i3d.3105

Pages: 326-332  |  89 Views  54 Downloads

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