The study was conducted at the Laboratory of the Department of Zoology, B. N. Mandal University in Madhepura, Bihar, India. The experiment was carried from May 2019 to April 2021. The average Total Bacterial Count (TBC) in the control water tank was 2.7 while the average TBC in treatment tank T₁ was 3.9. The average TBC in treatment tank T₂ was also 3.90. This suggests that treatment tank T₂ had a similar average total bacterial count as treatment tank T₁. In fish samples the TBC value were 0.76, 1.09 and 1.23 for T₀, T₁ and T₂ respectively. The average Total Coliform Count (TCC) in fish in the control tank was 0.29. The average TCC in treatment tank T₁ was 0.53. Compared to the control tank, treatment tank T₁ fish had a higher average total coliform count. The average TCC in treatment tank T₂ was 1.23 with a standard deviation of 0.08. Treatment tank T₂ had the highest average total coliform count among the three treatment tanks.