A 60 day feeding experimental trails were conducted to evaluate the effect of selected carbohydrate sources on growth performance in white shrimp L. vannamei under Biofloc system. The present set of Feeding trails consists of 8 Experimental groups with different Carbon sources in combinations. 1^st group with Control diet, 2^nd group with Control feed with Probiotics added, 3^rd group Molasses & Tapioca, 4^th group Molasses & Maize flour, 5^th group Molasses & Sucrose, 6^th group Molasses, Tapioca & Maize flour, 7^th group Molasses, Maize flour & Sucrose and 8^th group Molasses, Maize flour, Tapioca & Sucrose as Carbon sources in combinations. In the present study both Probiotics and combinations of Carbon sources used for the production of Bioflocs in the culture medium. The microbial community produced serve as additional food in addition to the regular feed supply for shrimp, produces relatively higher Growth rates and finally Productivity rates. The combined use of both Probiotics and BFT, generally acts as efficient alternative against pathogens and maintenance of ideal Water quality parameters, which in turn substantially enhances the growth potentials of shrimp L. vannamei. So the Probiotics selected in the present study Bacillus licheniformis and Lactobacillus rhamnosus and Bioflocs produced by combinations of molasses, tapioca, maize flour and sucrose yielded the best and highest Growth potentials in L. vannamei. Among the Experimental diets, Experimental diet (ED-7) formulated with both Probiotics and Bioflocs developed by the addition of Molasses, Tapioca, Maize flour and Sucrose yielded highest growth rates in L.vannamei.