The present investigation was conducted in Dzuna river of Jotsoma village under Kohima district of Nagaland, India to study the aquatic fauna biodiversity in between 2020 to 2021. During the survey a total of 926 individuals representing 37 species belonging to 6 classes, 13 orders, 22 families were recorded. Maximum of 36 species of aquatic fauna were recorded in Lieho (upstream) and minimum of 33 species were recorded in Chiiriizhu (downstream). At family level, Heptageniidae dominated the composition by 7%, Cyprinidae by 3%, Aeshnidae, Libellulidae, Perlidae, Ranidae by 2% and others by 1% each in composition. Species belong to order Diptera showed the highest relative abundance by 0.777 while lowest relative abundance was showed by order Decapoda with 0.001. Maximum diversity (Hs=3.25) and minimum diversity (Hs=3.22) was recorded in Lieho and Chiiriizhu accordingly. Maximum dominance Ds=0.95 and the least Ds=0.98 was recorded in Dziimetou and Chiiriizhu respectively.