The study evaluated the present scenario and effectiveness of the prevailing systems of biosecurity policy in microalgae culture premises of different shrimp hatchery in Cox’s Bazar, Bangladesh. The knowledge, attitude and practice (KAP) survey tool was used to assess the effectiveness of the biosecurity measures adopted by different stakeholders with the participation of 65 individual technicians’ from 27 different shrimp hatcheries. The KAP result indicates significant attitudes and practices gap among the technicians’. But the knowledge and attitudes scores were good (78.1% & 76.4%) where the practice scores were fair 63.5%. The survey data highlighted that the previous experience, educational level etc. have direct effect on KAP scores. However, there is no standardized systems are implementing in case of microalgae culture in hatchery premises. Through identifying some forthwith gaps, this work will help to identify all common problems and taking proper mitigation measures.