The lipid of muscle of the Bombay Duck (Harpadon neherus), collected from the economic zone Bay of Bengal, was extracted using a solvent extraction method and then characterized with regard to various physical and chemical properties and contrasted with those of standard lipids because marine fish lipids have grown in importance due to their positive effects on human health. In accordance with Gas Liquid Chromatography (GLC), the lipid sample contained 42.3412% Saturated Fatty Acids, 34.4958% Monounsaturated Fatty Acids (MUFA), and 23.2229% Polyunsaturated Fatty Acids (PUFA). Among PUFAs, percentages of ω-3 PUFAs (19.5245%) were higher than that of ω-6 PUFAs (3.6984%). The lipid sample's microbial activity was assessed (four bacteria were used for bacterial activity; four fungi were used for fungal activity). The titled specimen's lipid-containing muscle had undergone quantitative analysis to determine the percentages of its mineral contents (N, P, K, and Ca). Thus, it comes to a conclusion with a number of significant findings pertaining to industrial, pharmacological, and nutritional importance.