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E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2022, Vol. 10, Issue 2, Part A

Effect of semen cryopreservation with cucumber (Cucumis sativus) fruit juice (CJ) fortified- Extender on milt quality, viability and oxidative enzyme activity of Clarias gariepinus (Burchell, 1822)


Author(s): Chidobem IJ, Ezike CO, Nwamba HO and Uwadiegwu NC

Abstract: The present study investigated the quality, viability and oxidative enzyme activity of catfish semen fortified with cucumber fruit juice. The highest milt motility duration time of 66.6 seconds occurred among 20% CJ and was stored for 4 days. The lowest duration time of 50.3 s was recorded among the control group of milt stored for 2 days. The motility rate differed significantly between the control and CJ. The highest rate of 99.21% was recorded in control milt on day 4 compared with the least rate of 81.95% recorded among 15% CJ on day 1. The highest milt fertility rate of 75.35% was recorded in 10% CJ on day 2 compared with the least rate of 56.63% in control on day 1. The least hatchability rate of 64.21% was recorded in control day 3 compared with the highest value of 74.73% in 10% CJ on day 2. The highest milt survival rate of 99.46% was recorded among the control group on day 2, which compared with the least survival rate of 87.66% recorded on day 1 of 10, 15 and 20% CJ. There was a significant difference (P< 0.05) between the CAT control and treatments of CJ. The lowest value of 0.25 µmolemm-1 mg protein-1 recorded in control on day 1 compared with the highest value of 0.34µmolemm-1 mg protein-1 in 15% CJ on day 4. There was no significant difference in SOD activity between the control and CJ. Recorded values ranged between 0,026-0.56 Umole-1mgprotein-1 in 15% on day 2 and 10% day 1 respectively. There was no significant difference in LPO activity between the control and CJ. However, a range of 0.023-0.130 mMoleTbarsmm-1 protein-1 was recorded on day 1in 10% and control, respectively. 20 and 10% Cucumber fruit juice showed greater values in motility duration and fertility rate than control at respective storage periods for 4 and 2 days in Clarias gariepinus semen, and could in combination with DMSO improve its cryopreservation at short periods.

DOI: 10.22271/fish.2022.v10.i2a.2640

Pages: 08-16  |  125 Views  20 Downloads

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How to cite this article:
Chidobem IJ, Ezike CO, Nwamba HO, Uwadiegwu NC. Effect of semen cryopreservation with cucumber (Cucumis sativus) fruit juice (CJ) fortified- Extender on milt quality, viability and oxidative enzyme activity of Clarias gariepinus (Burchell, 1822). Int J Fish Aquat Stud 2022;10(2):08-16. DOI: https://doi.org/10.22271/fish.2022.v10.i2a.2640
International Journal of Fisheries and Aquatic Studies