Author(s): Rosidah, Muhammad Fathi Dhiaulhaq, Kiki Haetami and Roffi Grandiosa

Abstract: The research aims to determine the addition of herbal solutions on feed which could improve the immune system of Bonylip barb infected by Aeromonas hydrophila that indicated from the increased number of leukocyte and clinical signs. The method used in this research was Completely Randomized Design (CRD) with five treatments and three replications. The treatments are addition of herbal solutions on feed with the dose of 0 mL/kg (A), 100 mL/kg (B), 200 mL/kg (C), 300 mL/kg (D), 400 mL/kg (E). The treatments was maintainance for 21 days. At the end of maintenance, bonylip barb was infected by Aeromonas hydrophila with a density of 10⁸ cfu/mL. Observations number of total leukocytes counted before treatment, after treatment and after infected by Aeromonas hydrophila. The observations of clinical signs were damage surface of the body of fish test, shocks response and feeding response. The Data of total leukocytes count after treatment and after challenged were analyzed by ANOVA and followed by Duncan's test, while data of the clinical signs were analyzed descriptively. The results showed that the addition of herbal solutions concentrate as much as 200 mL/kg was the most effective to improve immune response of bonylip barb. During 21 days of treatments, total leukocytes count increased 36,92±2,34% from 44867 cell/mm³ to 71067 sel/mm³. Clinical symptoms were seen in the experiment from fish fed with addtional herb solution, however no significant damage was shown while recovery after experimental infections was faster compared with other treatments.

Pages: 234-240  |  359 Views  100 Downloads

Download Full Article: Click Here