Author(s): Velasco S Javier, Domínguez R María Guadalupe, García M Mauricio, Benítez A Andrea and Retana O Diego Alberto

Abstract: For the present investigation, the effect of a live food enriched with a probiotic was evaluated in 120 juvenile dregs of Cambarellus montezumae species with an average size and weight of 21 ± 2 (mm) and 0.490 ± 0.130 (g) respectively. Throughout the investigation, the organisms were kept in six translucent squared aquariums with 40 L of fresh water, at a temperature of 24 ± 1 °C, with a natural photoperiod and with 20 bowls each. The specimens were fed for 24 weeks with Tubifex tubifex slimeworm inoculated with 1 X 10⁷ CFU/g with the probiotic Spomune© (Bacillus subtilis and Clostridium butyricum). To verify the effect of the probiotic on the organisms, survival, growth (height and weight) as well as their degree of well-being were determined. The results show that the specimens fed with the enriched diet have a 95% survival compared to those fed with the unenriched diet was 48%. On the other hand, the growth showed significant differences between the two treatments (p <0.003) since the weight at the end of the experiment was 3.263 and 1.519 g with and without the incorporation of the probiotic in the food, respectively. In addition to this, the organisms fed without the probiotic presented a weight gain of only 0.774 g; while those fed the probiotic was 2.773 g. This knowledge generated allows to propose strategies for the conservation of the species, in the same way with this research the biological bases for a better cultivation in extensive, semi-intensive and intensive systems, under controlled conditions can be laid. This would reduce the pressure on this resource in its natural habitat, giving way to the recovery of natural populations and managing to meet the demand that this organism has at the local level and for aquarium purposes as live food.

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