Contact: +91-9711224068
International Journal of Fisheries and Aquatic Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2020, Vol. 8, Issue 4, Part B

Cloning and sequence analysis of Astacin like metalloproteinase gene from Indian oyster, Magallana bilineata (Röding, 1798)


Author(s): Esha Arshad, MA Pradeep, EA Nisha, TS Azhar Shahansha and KK Vijayan

Abstract: Astacins are a group of zinc-dependent metalloproteases with diverse roles in biological systems such as embryonic development, connective tissue remodelling, digestion, and processing of biologically active peptides. This paper describes the cloning of an Astacin like metalloproteinase (Mbi-ALMP) from the digestive tissues of Magallana bilineata. The Mbi-ALMP encodes a deduced 440 amino acid protein which includes an N-terminal signal peptide. The Mbi-ALMP possesses several of the characteristic features of the Astacin family like the pro-peptide domain, Astacin catalytic domain with Zinc binding motif, and met-turn, followed by a MAM (meprin, A5 protein, receptor protein-tyrosine phosphatase µ) carboxy-terminal domain. Amino acid sequence alignment and phylogenetic analysis of the protease domain indicate Mbi-ALMP to have a high degree of sequence homology to other Astacin like metalloproteases from different species.

Pages: 124-129  |  153 Views  11 Downloads

Download Full Article: Click Here

How to cite this article:
Esha Arshad, MA Pradeep, EA Nisha, TS Azhar Shahansha, KK Vijayan. Cloning and sequence analysis of Astacin like metalloproteinase gene from Indian oyster, Magallana bilineata (Röding, 1798). Int J Fish Aquat Stud 2020;8(4):124-129.
International Journal of Fisheries and Aquatic Studies