Author(s): Reyes Alvin T, Guinto Edgardo Jr. R and Doctolero Jemuel S

Abstract: The study was conducted in order to evaluate the antibacterial potential of acacia leaves, barks and roots aqueous extracts against the bacterium S. agalactiae by measuring the zone of inhibition and determining the minimum inhibitory concentration. Extract concentrations that recorded at least 50% survival on the bioassay of Nile tilapia fingerlings were used in the susceptibility test and these concentrations were: leaves = 0, 5 and 10 µg/µl; barks = 0, 5, 10, 20 and 40 µg/µl; and roots = 0 and 5 µg/µl. The zone of inhibition of leaves extract at 5 and 10 µg/µl was statistically significant as compared to 0 µg/µl. For barks extract, concentrations at 10, 20 and 40 µg/µl had significant higher zone of inhibition as compared to 0 µg/µl. Meanwhile, the zone of inhibition of roots extract at 5 µg/µl was significantly higher as compared to 0 µg/µl. The bacterium was still classified as resistant to the various extract concentrations based upon the Clinical and Laboratory Standards Institute (2012). The Minimum inhibitory concentration (MIC) result showed that acacia extracts from leaves, barks and roots were not capable of 100% inhibition of the bacterium. Even though the result was inconsistent, highest percent inhibition was recorded at 10 µg/µl for leaves, 5 and 20 µg/µl for barks and 400 µg/µl for roots.

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