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International Journal of Fisheries and Aquatic Studies
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E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2019, Vol. 7, Issue 2, Part D

Histology of gonads in Oreochromis niloticus (Linnaeus 1757) fed cottonseed meal-based diets

Author(s): Tope-Jegede OH, Fagbenro OA and Olufayo MO

Abstract: This study was conducted to evaluate the effect of cottonseed meal-based diets on histology of testes and ovaries of Oreochromis niloticus. Two hundred (200) apparently healthy Oreochromis niloticus, average body weight of (10g ± 0.20) were used for the investigation. The Fish were randomly distributed into five groups each at a rate of twenty (20) fish per treatment. Ten male and ten female fish were stocked in ten (10) concrete tanks (2m x 2m x 1m), each treatment was replicated twice. Five isoproteic diets were formulated at 37.5% crude protein. In the experimental diets, 0%, 25%, 50%, 75%, and 100% of soybean meal protein were substituted with cottonseed meal. The fish were fed at 5% body weight twice daily for 90 days. At the end of the 90 days, the testes and ovaries were sectioned for histological examination. At the end of the experiment, it is only in the control diet (0g CSM/kg diet), that the histological section of the testes of O. niloticus showed normal sperm cell distribution and abundant spermatozoa in the seminiferous cavities with no visible lesion. In all the other cottonseed meal-based diets, there were numerous cavities where spermatocytes appeared scanty, scanty lumina of the seminiferous tubules, thick and reduced interstitium/sertoli cyst and reduced lumen size. Cottonseed in the diets was able to destroy the spermatocytes and therefore, there was no reproduction at all in the other cottonseed meal-based diets. Histological section of ovary from O. niloticus fed 0g/CSM/kg diet (control) showed normal ovary architecture and the vitellogenic stages, no visible lesion seen. In all the cottonseed meal-based diets, O. niloticus ovary showed distorted vitellogenic stages and other stages still in their early phases of development. The damage done to the ovaries could not allow them to complete the reproductive cycle, the eggs could not be hatched and there was no reproduction.

Pages: 269-274  |  364 Views  17 Downloads

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How to cite this article:
Tope-Jegede OH, Fagbenro OA, Olufayo MO. Histology of gonads in Oreochromis niloticus (Linnaeus 1757) fed cottonseed meal-based diets. Int J Fish Aquat Stud 2019;7(2):269-274.
International Journal of Fisheries and Aquatic Studies