Author(s): Harton Arfah, Agus Oman Sudrajat, Muhammad Agus Supriyadi and Muhammad Zairin

Abstract: Reproductive engineering for gonadal maturation process is mostly related with four factors such as, environmental signals, environmental signals, reproductive organs, hormonal and nutrition. This research was conducted to obtained mature gonad of Striped Catfish, Pangasianodon hypophthalmus utilized three kinds of materials with eight treatments which is (PMSG hormone + Antidopamine), Vitamin E, and Turmeric. While, the experimental designed consisted of K (Control), HEKu (Hormon + Vitamin E + Turmeric), HE (Hormone + Vitamin E), HKu (Hormone + Turmeric), Eku (Vitamin E + Turmeric), H (PMSG + Antidopamine), E (Vitamin E), and Ku (Turmeric) with egg (5%) to the feed as a binder. This research was done by feeding fish with treated feed for twelve weeks. Fish growth showed no significant difference between treatments (P>0.05), but EKu treatment had the lowest growth. Fish hepatosomatic and gonadosomatic indices showed no difference before treated, but showed variation after 6 weeks of feeding.. Egg diameters were increased in line with the rearing duration. K and H treatment has the highest estradiol concentration in week-12. Testosterone and estradiol concentration had two peaks in all treatment except HKu which had three peaks at two, six, and ten weeks of rearing. Histological analysis showed that feeding with combination of hormone and turmeric (HKu) gave faster gonad development than other treatment. The result of this research concluded that (PMSG + Antidopamine) and turmeric in feed was able to accelerate the gonadal maturation in stripedcatfish.

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