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International Journal of Fisheries and Aquatic Studies
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(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2018, Vol. 6, Issue 4, Part D

Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish (Pangasius hypophthalmus)


Author(s): Divya K Vijayan, Sreerekha PR, Tejpal CS, Asha KK, Mathew S, Ravishankar CN and Anandan R

Abstract: The aim of the present study was to extract high pure acid soluble collagen (ASC) from the airbladder of striped cat fish (Pangasius hypophthalmus). ASC was extracted and its physico-chemical characterization was carried out to confirm the purity and structural integrity of extracted collagen. The yield of ASC extract was determined to be 73.4±0.8% of the dry weight of raw material. Amino acid analysis revealed the glycine content as 243.0±1.3 residues/1000 residues, imino acids as 186.1±1.4 residues/1000 residues respectively and was shown to be devoid of non-collagenous amino acids such as tryptophan. Electrophoretic analysis confirmed the subunit pattern of type I collagen and showed that it also contained β and ɤ subunits. UV/Vis absorption and FT-IR analysis demonstrated the primary and secondary structural integrity of collagen and also indicated that no denaturation had occurred during extraction process. 1H-NMR analysis validated the structural integrity of collagen triple helix. The denaturation temperature (Td) of ASC was assessed as 33.3 °C. The air bladder collagen extracted from striped cat fish is of high purity and belongs to type I with intact triple helical structure.

Pages: 310-318  |  934 Views  32 Downloads

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How to cite this article:
Divya K Vijayan, Sreerekha PR, Tejpal CS, Asha KK, Mathew S, Ravishankar CN, Anandan R. Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish (Pangasius hypophthalmus). Int J Fish Aquat Stud 2018;6(4):310-318.
International Journal of Fisheries and Aquatic Studies