International Journal of Fisheries and Aquatic Studies
2018, Vol. 6, Issue 4, Part A
Isolation and identification of zoonotic bacteria, Proteus penneri from a disease outbreak in goldfish, Carassius auratus in KeralaAuthor(s):
Wafha KA, Arathi Dharmaratnam, Raj Kumar, VS Basheer and T Raja SwaminathanAbstract:
Moribund goldfish, Carassius auratus
, from a local ornamental fish farm with 50% cumulative mortality was sampled with the aim of isolating and detecting the causative agentin Ernakulam, Kerala. Five bacterial species viz., Enterobacter cloacae
, Klebsiella pneumoniae, Bacillus cereus, Aeromonas hydrophila
and Proteus penneri
were isolated, and identified on the basis of biochemical tests and sequencing of 16S rDNA gene using universal bacterial primers. Challenge experiments with these isolates using healthy goldfish suggested that P. penneri
induced show similar clinical and pathological states within 5 days of injection and confirmed that P. penneri
was the causative agent.
Kanamycin (30µg/disc), Ciprofloxacin (30µg/disc), Cefixime (5µg/disc) and Enrofloxacin (10µg/disc) are sensitive to the pathogen identified by the antibiotic sensitivity test. This study also assessed the toxic activities of extracellular products from P. penneri
using in vitro
assays on CCKF cell line. The affected fish recovered from the infection after treatment with the suggested antibiotics.Pages: 24-30 | 496 Views 30 DownloadsDownload Full Article:
How to cite this article:
Wafha KA, Arathi Dharmaratnam, Raj Kumar, VS Basheer, T Raja Swaminathan. Isolation and identification of zoonotic bacteria, Proteus penneri from a disease outbreak in goldfish, Carassius auratus in Kerala. Int J Fish Aquat Stud 2018;6(4):24-30.