International Journal of Fisheries and Aquatic Studies
2018, Vol. 6, Issue 2, Part H
Toxicity of aqueous extracts of bitter leaf (Vernonia amygdalina) on haematological profile of African catfish (Clarias gariepinus) juveniles
Author(s): Olowolafe T and Olufayo MO
Abstract: The toxic effects of the aqueous extracts of
Vernonia amygdalina on
Clarias gariepenus were investigated under laboratory conditions a 96h exposure period using blood profile and behavioral responses as biomarkers of environmental quality in toxicity testing. One hundred and eighty (180) healthy juveniles of
C. gariepinus (17.05±0.25g) were acclimatized for a week, weighed and randomly distributed into eighteen bioassay tanks (20L) with stocking density of ten (10) fish per tank. A range finding test was conducted prior to the experiment. The fish were later exposed to 0ml (contol), 40ml, 45ml, 50ml, 55ml, and 60ml per litre concentrations of aqueous extracts of
V. amygdalina for 96hrs in a static bioassay procedure. The erratic swimming led to loss of equilibrium, respiratory disturbance, lethargies and mortality of the text organisms which then increased significantly with increasing concentrations of plant extract. Differences observed in the mortalities of
C. gariepenus at varying concentrations were significant (P < 0.05). Hematological indices indicated a significant (P < 0.05) decreasing trend in the red blood cell, haemoglobin and the packed cell volume with increasing concentrations of
V. amygdalina while the leucocyte counts increased with increasing concentrations of
V. amydgalina. The 96h LC50 was determined as 53.52ml/l.
Pages: 596-600 | 1614 Views 863 DownloadsDownload Full Article: Click Here
How to cite this article:
Olowolafe T, Olufayo MO. Toxicity of aqueous extracts of bitter leaf (Vernonia amygdalina) on haematological profile of African catfish (Clarias gariepinus) juveniles. Int J Fish Aquat Stud 2018;6(2):596-600.
