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International Journal of Fisheries and Aquatic Studies
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(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2018, Vol. 6, Issue 2, Part G

Effect of two probiotics on bacterial community composition from biofloc system and their impact on survival and growth of tilapia (Oreochromis niloticus)


Author(s): Cienfuegos Martínez Kathia, Monroy Dosta María del Carmen, Hamdan Partida Aida, Castro Mejía Jorge, Aguirre Garrido José Félix and Bustos Martínez Jaime Amadeo

Abstract: The main goal of this study was to evaluate the effect of two probiotics on bacterial community composition of Biofloc system using molasses as carbon source. 135 tilapia organisms with an initial mean weight of 7.08±0.17g and a total length of 7.47±0.16cm were used for the present study. The fish were cultivated in plastic containers of 100L testing three experimental diets: Biofloc as control, Biofloc enriched with Bacillus subtilis and Biofloc enriched with Rhodococcus sp. Every 15 days, weight and length, as well as water quality parameters were analyzed. For DNA extraction, 1L of water was extracted from each treatment (initial, middle and final phase). The samples were sent to IMR to make the massive sequencing of the region V4-V5 from 16S rRNA. The bioinformatic analysis was made with MOTHUR and STAMP programs. 20 phyla were obtained, from which the most abundant were: Proteobacteria, Bacteroidetes, Actinobacteria, Planctomycetes, Verrucomicrobia and Fusobacteria from each treatment. Bacterial community showed variations in different periods, at the beginning were similar in all the cases, while at the end Rhodococcus sp. was different because it showed variations in the composition. The probiotics did not show significant effects in growth but improved the tilapia organism’s survival.

Pages: 525-533  |  735 Views  99 Downloads

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How to cite this article:
Cienfuegos Martínez Kathia, Monroy Dosta María del Carmen, Hamdan Partida Aida, Castro Mejía Jorge, Aguirre Garrido José Félix, Bustos Martínez Jaime Amadeo. Effect of two probiotics on bacterial community composition from biofloc system and their impact on survival and growth of tilapia (Oreochromis niloticus). Int J Fish Aquat Stud 2018;6(2):525-533.
International Journal of Fisheries and Aquatic Studies