International Journal of Fisheries and Aquatic Studies
2018, Vol. 6, Issue 2, Part G
Detection and typing of Blastocystis spp. in oysters (Crassostrea virginica) collected in Actopan River, Chachalacas, Veracruz
Author(s): José Mijail Campos Compean, Jaime Bustos-Martínez, Ignacio Barbabosa Martínez, María del Carmen Monroy Dosta and Aida Hamdan-Partida
Abstract: The main goal of this study was to determine the presence and
Blastocystis spp subtype in oysters (
Crassostrea virginica) collected at Actopan River, Veracruz. Three samplings were made during one year at the principal oyster collection areas. The oysters were dissected to obtain their gut, which was inoculated in
Blastocystis spp. culture medium and incubated for seven days to analyze them by microscopy technique. Samples that were positive to
Blastocystis spp. their DNA was extracted by PCR technique and amplified ITS1–5.8S–ITS2 region of 18S rDNA gen. The amplicons were sequenced and subsequently were typing using bioinformatic program. It was found that
C. virginica show a high prevalence of
Blastocystis spp., 67% were positive and it was determined that
Blastocystis spp. subtype isolated from
C. virginica was subtype 1 (ST1).
Pages: 511-514 | 1169 Views 172 DownloadsDownload Full Article: Click Here
How to cite this article:
José Mijail Campos Compean, Jaime Bustos-Martínez, Ignacio Barbabosa Martínez, María del Carmen Monroy Dosta, Aida Hamdan-Partida. Detection and typing of Blastocystis spp. in oysters (Crassostrea virginica) collected in Actopan River, Chachalacas, Veracruz. Int J Fish Aquat Stud 2018;6(2):511-514.
