Author(s): Ashvani Kumar Srivastav and Dayalanand Roy

Abstract: Fish farmers have widely used malachite green, as an antifungal, antibacterial, antiparasitical and antiprotozoal agent. It is also used as dye on silk, wool, jute, leather, cotton and paper industries. Through the use of this dye has been banned in several countries, due to its low cost, and due to proper alternative, it is still being used in many parts of the world. Recently a pharmaceutical alternative to malachite green, pyceze as its active ingredient has been developed at U. K. It is being used for the treatment of fish fungal infection and their ova and appears to be a safe and effective replacement. Fish were exposed for four days to 1/5^th of LC50 values of each of malachite green (0.24 mg/l) and (0.42 mg/l). After four days blood glucose level exhibited significantly increased whereas liver glycogen and muscle glycogen were decreased in fish in both groups when compared with controls. The present study evaluvated the effect of malachite green and pyceze on carbohydrate metabolism in Heteropneustes fossilis. However, exposure to malachite green was found to be more toxic than to pyceze.

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