International Journal of Fisheries and Aquatic Studies
2018, Vol. 6, Issue 1, Part A
DNA barcoding and molecular population structure of two species from genus Diplodus based on COI gene in the Egyptian Mediterranean Sea
Author(s): Eman M Abbas, Engy T Megahed, Shabaan A Hemeda and Abeer F. ElNahas
Abstract: Genetic population structure and DNA barcoding of two species
Diplodus, (
Diplodus sargus and
Diplodus vulgaris) belonging to family Sparidae, were determined by using 652 bp of the mitochondrial DNA sequences of the COI gene for 114 individuals collected from six locations in the Egyptian Mediterranean Sea. Eleven haplotypes were detected for
D. sargus including 10 polymorphic sites and 10 haplotypes for
D. vulgaris containing 16 polymorphic sites without insertion and deletion. Two dominant haplotypes of
D. sargus were shared among the six locations, while only one haplotype of
D. vulgaris was common in the same mentioned locations. The haplotype and nucleotide diversities of
D. sargus individuals were 0.652 and 0.00134 respectively, while those of
D. vulgaris were0.541 and 0.00188, respectively. There is no significant population subdivision between all six locations based on the
Fst values (genetic diversity) of the population for the two species from genus
Diplodus. Therefore, our data reflects the high connectivity between all populations indicating that these populations share the same genetic material.
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How to cite this article:
Eman M Abbas, Engy T Megahed, Shabaan A Hemeda, Abeer F. ElNahas. DNA barcoding and molecular population structure of two species from genus Diplodus based on COI gene in the Egyptian Mediterranean Sea. Int J Fish Aquat Stud 2018;6(1):01-08.