International Journal of Fisheries and Aquatic Studies
2017, Vol. 5, Issue 5, Part E
Cryogenic preservation of critically endangered Cirrhinus reba fish spermAuthor(s):
Shirin Sultana, Md. Mahmud Hasan, Mohammad Shahdat Hossain, Md. Rashidul Islam, Md. Salimullah, Md. Rafiqul Islam Sarder, Md. Saidul Islam, Md. Moniruzzaman and Jahangir AlamAbstract:
The natural population of Cirrhinus reba
in Bangladesh has been decreasing rapidly and is subsequently categorized as threatened. The study was carried out to develop a suitable protocol for C. reba
sperm cryopreservation. The sperm activation was observed in 0.1%-1.1% NaCl solution with highest motility 92.5 ± 2.5 (mean±SE) at 0.3% NaCl solution and lowest (zero motility) at 1.1%. Two different extender such as inorganic chemical Alsever’s solution, organic egg-yolk citrate and two cryoprotectants namely methanol and dimethylsulfoxide (DMSO) were used in toxicity analysis at 5%, 10% and 15% concentration.
The cryopreservation was carried out with three diluents (Alsever’s solution plus 10% DMSO, Alsever’s solution plus 10% methanol; Egg-yolk citrate plus 5% DMSO) in two steps freezing protocol (from room temperature to -4°C at a rate of 4°C per min and then from -4°C to -80°C at a rate of 10°C per min). Alsever’s solution with 10% DMSO showed best performance and producing 85±5.77 and 58.75±3.15% equilibration and post-thaw motility, respectively. This protocol will be helpful to conserve the gene pool of C. reba
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How to cite this article:
Shirin Sultana, Md. Mahmud Hasan, Mohammad Shahdat Hossain, Md. Rashidul Islam, Md. Salimullah, Md. Rafiqul Islam Sarder, Md. Saidul Islam, Md. Moniruzzaman, Jahangir Alam. Cryogenic preservation of critically endangered Cirrhinus reba fish sperm. Int J Fish Aquat Stud 2017;5(5):334-339.