Author(s): Mary Shilma, Dr. K Dinesh, Dr. PM Sherief, Dr. S Shyama, Dr. Devika Pillai and V Malika

Abstract: An unidentified rare mahseer species of the Chalakudy river is a red variety locally known as ‘choppan choora’ is not eaten by the people as its consumption leads to giddiness, nausea and vomiting which last for at least 24 hours. Bioassay-guided fractionation was employed to identify the active compounds from fish sample. Purification of the crude extract was done by Gel filteration chromatography. Spectrophotometric analysis of Tor sp. showed highest peaks as compared to the Tor khudree sykes, 1839. Water sample analysis of toxic cyanobacteria showed negative result by PCR. Preliminary studies on mouse Bioassay was carried out at Central Institute of Fishery Technology, Kochi. Fraction showed mild symptoms in Aqueous extract. Intraperitoneal mice study was conducted at Kerala Veterinary and Animal Sciences University, Thrissur. Mice exhibited lethality within 24hr. Control was maintained throughout the study from non toxic Tor sp.

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