Author(s): Muhammad Faiz Islami, Agus Oman Sudrajat and Odang Carman

Abstract: There are constraints on the production of red fin shark Epalzeorhynchos frenatus due to the lack of matured broodstocks in Non-spawning season and has a dependence on hormone induction during spawning process, because it can’t spawn naturally in cultivation environments. Gonad maturation can be stimulated by the addition of hormones and potential plant-based materials to feed. Thus, the present study attempted alternative hormonal combination to induce both ovulation and spawning in order to provide farmers with alternative choices for reproduction. This research was divided into two sub-research (maturation and ovulation) in one main title. The first section of the study dealt with maturational induction and it used Completely Randomized Design with 5 treatments. Ten broodstocks were used in each treatment which was as follows: Oodev (0.25 and 0.5 mL. kg-1 fish) addition to feed, turmeric powder (250 and 500 mg 100 g-1 feed), and commercial feed (at least 30 % protein) as the control. Broodstocks with 20.61±0.57 g fish-1 of weight were maintained for 56 days. Spawning was done by using ready-spawning broodstock with a ratio of male : female (3:1), which consisted of 4 treatments, namely Spawnprim (0.5 and 1.0 mL. kg-1 fish), 0.5 mL Ovaprim kg-1 fish and 0.9 % NaCl solution as positive and negative control. The addition of 0.5 mL Oodev kg-1 fish and 500 mg 100 g-1 turmerics respectively was able to produce matured broodstocks amounted to 50 % and 20 % of population, whereas matured broodstocks were not found in control. Spawnprim induction achieved 100% spawning rate, the same result was found on Ovaprim induction (P>0.05). The results of this study were expected to be a reference that mature broodstock should be available throughout the year and it is required to provide an alternative spawning hormone.

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