International Journal of Fisheries and Aquatic Studies
2017, Vol. 5, Issue 4, Part F
Isolation and identification of Myxobolus cerebralis from brain of Heteropneustes fossilis in the beels of South 24-Parganas, West Bengal, India
Author(s): Ananya Guchhait, Koel Bhattacharya Sanyal, Debapriyo Mukherjee, Prasenjit Mali, TJ Abraham and Gadadhar Dash
Abstract: Myxobolus cerebralis infecting the brain of Heteropneustes fossilis (singhi) was identified morphologically, histopathologically and by molecular method captured from the beels of Raidighi, South 24 Parganas, West Bengal, India. Infection of M. cerebralis was uncommon in Heteropneustes fossilis brain. The infection rate was low to moderate. Microscopically Myxobolus parasites were detected in brain of Heteropneustes fossilis (singhi). Large plasmodia were localized within brain, causing severe necrotic changes and vacuolization. Inflammatory infiltrates agglomeration, nuclear hypertrophy, vacuolization, infiltration of blood cell and haemorrhages were extensively observed in the brain. Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify an approximately 1600 base pairs (bp) long fragment of the 18S ribosomal RNA gene.
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How to cite this article:
Ananya Guchhait, Koel Bhattacharya Sanyal, Debapriyo Mukherjee, Prasenjit Mali, TJ Abraham, Gadadhar Dash. Isolation and identification of Myxobolus cerebralis from brain of Heteropneustes fossilis in the beels of South 24-Parganas, West Bengal, India. Int J Fish Aquat Stud 2017;5(4):413-417.