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International Journal of Fisheries and Aquatic Studies
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E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2017, Vol. 5, Issue 4, Part E

Isolation and characterization of probiotic bacteria from the soil samples of the coastal areas of (Gudur division, Nellore Dt.) for utilization in Shrimp farming


Author(s): K Lavanya and Y Dayakar

Abstract: A study was conducted to isolate and characterize probiotic bacteria from soils of different areas in Nellore district for utilization in Shrimp farming. Conventional, morphological and biochemical tests were conducted to isolate and enumerate the Lactobacillus bacteria from the soil samples. Bacterial growth kinetics, tolerance to various parameters like ammonia, nitrogen, salinity, pH and bile acid were taken into consideration to characterize the isolated bacteria as probiotic. Cholesterol assimilation and viability of bacteria in gastric tract was also studied. Bacterial DNA samples isolated were found to have the V2-V3 sequence of lactobacillus which is of approximately 200bp fragment). All the screened samples were shown to be amplified for V2-V3 region except DZ1 and DZ6.

Pages: 371-376  |  486 Views  11 Downloads

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How to cite this article:
K Lavanya, Y Dayakar. Isolation and characterization of probiotic bacteria from the soil samples of the coastal areas of (Gudur division, Nellore Dt.) for utilization in Shrimp farming. Int J Fish Aquat Stud 2017;5(4):371-376.
International Journal of Fisheries and Aquatic Studies