International Journal of Fisheries and Aquatic Studies
2017, Vol. 5, Issue 4, Part D
Effects of Nano-coating on biofilm forming bacteria on different boat building materials using 16sr DNAAuthor(s):
Archana S, Sundaramoorthy B, Neethiselvan N and Jeyashakila RAbstract:
The present study was aimed to see the effect of nano coating on biofilm formation in different boat building materials. Test panels (15cm h x 8 cm b and 12 mm thickness) made up of selected boat building materials (Steel, Wood, FRP) were tied in an iron frame and hung at a depth of 1.5 m inside the Tuticorin New Port area. The synthesized copper nano powder was mixed with enamel paint and spray coating was done on different boat building material test panels under room temperature. Periodical sampling was done to study the biofilm forming bacteria. Total counts of viable bacteria in untreated controls increased with time. From the biofilm forming bacteria, morphologically dissimilar colonies were randomly selected, isolated and maintained in Zobell Marine slants. A total of four isolates were selected from the test panels (H1, A2, A3, and A4). The selected four isolates were amplified using the primer specific for mt 16S rDNA gene. The first isolate was identified as Pseudomonas aeruginosa (H1) based on 99% similarity. The third isolate was Vibrio alginolyticus (A3). These two species were present in all type of panels. The second isolate was identified as Ferrimonas futtsuensis (A2), which was present in steel control and nano coated panels. The fourth isolate was Vibrio coralliilyticus (A4) which was present in FRP panels and wooden panels. The phylogenetic analysis was preferred with the sequence of four isolates taken in this study and twelve biofouling bacterial sequences already reported by the authors in the previous works.Pages: 300-307 | 566 Views 13 DownloadsDownload Full Article:
How to cite this article:
Archana S, Sundaramoorthy B, Neethiselvan N, Jeyashakila R. Effects of Nano-coating on biofilm forming bacteria on different boat building materials using 16sr DNA. Int J Fish Aquat Stud 2017;5(4):300-307.