Author(s): Lydia Booney Jyrwa and Rabindra Nath Bhuyan

Abstract: The Chocolate Mahseer (Neolissochilus hexagonolepis McClelland, 1839) is an important food and sport fish of the region and its population is sharply declining due to various anthropogenic activities. However, for culture and conservation of the fish, there is no induced breeding protocol so far for the agro-climatic condition of Meghalaya. Thus it becomes necessary for standardized of doses of pituitary as well as synthetic hormone for artificial propagation of the fish and develop an induced breeding protocol. The fish was successfully bred at the Hatchery Complex, St. Anthony’s College, Shillong, Meghalaya by using pituitary extract and synthetic hormone viz. ovaprim and gonopro-FH. The dose of pituitary extract is standardized as 4 mg/kg body weight to both male and female as 1^st dose and at 7.9 mg/kg body weight only to female as 2^nd dose. For ovaprim, a single dose as 0.8 ml/kg body weight to female and 0.3 ml/kg body weight to male for ovaprim was given and as 0.9 ml/kg body weight to female and 0.3 ml/kg body weight to male for gonopro-FH. The fecundity of the fish was 3500 eggs/kg body weight which was comparatively low. The final hatching percentage achieved was 60% and survival rate of hatchling was 50% up to fry stage.

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