Author(s): Hany MR Abdel-Latif and Eman Khalifa Sedeek

Abstract: Enterobacteriaceae encompasses a group of fish pathogens that frequently occurred as water-borne infections and may be present in the tissues of apparently healthy fish and remain opportunistic in nature. Full clinical, bacteriological and phenotypic characterizations were done on two hundred of diseased Oreochromis niloticus, from some private farms from Beheira governorate during summer 2015. Prevalence% of the identified bacterial isolates and their incidence in the internal organs of the diseased fish were determined. Additionally, Antimicrobial sensitivity testing for the most prevalent isolates was also established using 11 antimicrobial discs. It was found that the infected fish have general septicemic signs like skin hemorrhages and ulcerations with noticeable focal hemorrhages and areas of necrosis in liver and congestion in gills and spleen. About 144 Enterobacteriaceae strains were retrieved from diseased fish. The identified strains were: Citrobacter freundii, Edwardsiella tarda, Enterobacter aerogenes, Enterobacter cloacae, Escherichia coli, Hafnia alvei, Klebsiella sp, Morganella morganii, Proteus vulgaris, Proteus mirabilis, Providencia sp, Salmonella sp, Serratia marcescens and Shigella sp, with average prevalence% were 25.69, 10.42, 6.25, 11.81, 15.28, 4.17, 7.64, 4.86, 3.47, 1.39, 1.39, 1.39, 4.17 and 2.08 respectively. The results showed that the maximum number of isolates was claimed from liver (49.31%) and the lowest number of isolates was obtained from the kidney (13.19%). It was found that the bacterial isolates were markedly sensitive to Ciprofloxacin, gentamycin, Florfenicol, and Flumequine and moderately sensitive Doxycycline and less sensitive to Oxytetracycline, Erythromycin, and Ampicillin. The findings highlight the extreme diversity of Enterobacteriaceae that are potentially associated with fish diseases in Egypt.

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