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International Journal of Fisheries and Aquatic Studies
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(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2017, Vol. 5, Issue 1, Part A

Molecular characterization and expression analysis of gonadotrophic α-subunit hormone in rohu (Labeo rohita)


Author(s): Avinash Pradhan, Suresh Chandra Rath and Ashis Saha

Abstract: Reproduction in fish is controlled by two pituitary gonadotropin hormones, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Rohu (Labeo rohita) is a commercially important seasonal breeder freshwater fish species, but the information regarding molecular regulation of reproduction in this important carp species is very scanty. In the present study we identified, cloned and characterized gonadotropin α-subunit (GTHα) full-length cDNA by RACE (rapid amplification of cDNA ends), and analyzed their basal expressions in different tissues by quantitative real time PCR (qRT-PCR) assay. Full-length rohu GTHα cDNA encodes 118 amino acids and in the adult fish, they were widely expressed in pituitary, brain, gonads, muscle, liver, intestine, gill, kidney, eye and heart.

Pages: 23-28  |  794 Views  40 Downloads

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How to cite this article:
Avinash Pradhan, Suresh Chandra Rath, Ashis Saha. Molecular characterization and expression analysis of gonadotrophic α-subunit hormone in rohu (Labeo rohita). Int J Fish Aquat Stud 2017;5(1):23-28.
International Journal of Fisheries and Aquatic Studies