International Journal of Fisheries and Aquatic Studies
2016, Vol. 4, Issue 6, Part C
Comparative isolation and amplification of Cytochrome oxidase 1 DNA from Oncorhynchus mykiss (Rainbow Trout) of Azad Jammu & Kashmir
Author(s): Nuzhat Shafi, Abdul Rauf, Tasleem Akhtar, Riaz Aziz Minhas, Ulfat and Sidra Bibi
Abstract: DNA extraction plays a pivotal role in the polymerase chain reaction (PCR) based molecular and genetic characterization methods. In order to find out the exact genetic lineage of the trout fish found in AJK, the fish DNA was extracted and analyzed further. Due to complicatedness regarding the fish DNA extraction, three different extraction methods were employed. We extracted genomic DNA from Rainbow trout by using standard phenol Chloroform method, a modified phenol Chloroform method and DNAzol DNA extraction method. The yield and quality of DNA extracted through different methods was compared. It was found that the extraction made by treating the samples with sucrose during initial steps of DNA extraction (Sucrose, Tris HCl (pH 7.5), MgCl
2, 1%V/V Triton, SDS and proteinase K) showed the best results while DNAzol method was the least productive. Likewise the modified method was also found to be the most productive in terms of PCR yield of
cytochrome oxidase 1 gene.
Pages: 196-199 | 1170 Views 88 DownloadsDownload Full Article: Click Here
How to cite this article:
Nuzhat Shafi, Abdul Rauf, Tasleem Akhtar, Riaz Aziz Minhas, Ulfat, Sidra Bibi. Comparative isolation and amplification of Cytochrome oxidase 1 DNA from Oncorhynchus mykiss (Rainbow Trout) of Azad Jammu & Kashmir. Int J Fish Aquat Stud 2016;4(6):196-199.
