International Journal of Fisheries and Aquatic Studies
2016, Vol. 4, Issue 5, Part H
Molecular cloning and computational characterization of thymidylate synthase in white spot syndrome virus (WSSV)Author(s):
Vaishnavi Ramasubbu, Amrendra Kumar and A SaravanakumarAbstract:
White spot Syndrome (WSS) still remains a major challenge for Biologist due to its huge devastation in major sea food items especially shrimps. Several studies had been carried out on white spot syndrome virus (WSSV) genome as well as host genomes. Among all, the thymidylate synthase (TS) is a one of the major target enzyme remains to be studied for cancer and other viral disease. Hence the present study is a mile stone to study the basic properties of WSSV thymidylate synthase. In this study, WSSV TS was amplified by using specific primer and cloned into pTZ vector by T tail ligation and further sub-cloned into pRSET-B vector. Physico-chemical properties of amino acid, multiple sequence alignment, phylogenetic tree analysis, phosphorylation site and transmembrane region were predicted; moreover 3 D modeling of WSSV TS has been studied by using bioinformatics tools. The amplified product from cDNA was 867 bp and was confirmed by agarose gel, which gives 289 residues of amino acid. Among them serine (s) 40 (0.997) was found as a more active phosphorylation site, 201-205 amino acid was potential to be in transmembrane region. Multiple sequence alignment shows its conserved domain in all respective species by their similarities and phylogenetic analysis revealed WSSV TS CDS is maximum similar to human TS (67%). The orientation of homo-dimer protein structure was found. This study is essential for basic knowledge development of WSSV TS activity which will lead us to identify or develop a strong inhibitor in near future.Pages: 557-563 | 744 Views 20 DownloadsDownload Full Article:
How to cite this article:
Vaishnavi Ramasubbu, Amrendra Kumar, A Saravanakumar. Molecular cloning and computational characterization of thymidylate synthase in white spot syndrome virus (WSSV). Int J Fish Aquat Stud 2016;4(5):557-563.