Author(s): Sreenivasulu P, Suman Joshi DSD, Narendra K, Venkata Rao G, Krishna Satya A

Abstract: The present study was primarily based up on testing and evaluation of the bacteria Bacillus pumilus. It was used as a feed supplement in the diet for Litopenaeus vannamei (L. vannamei), which were left to be acclimated to laboratory conditions for a period of one week. Shrimp were divided into 4 groups consisting of 6 shrimp and all the experiments were run in triplicate. Three diets were formulated containing different doses (5%, 10% and 15%) of the probiotic bacteria. Keeping one group as control and the remaining three groups were fed with probiotic supplemented feed (1X106 at10g gel/kg feed, 50gms, 100g, 150g of gel was added per Kg feed to make 5%, 10% and 15%). After feeding the shrimp with probiotic bacteria, different parameters such as antagonistic property, growth performance and hemolymph parameters such as the total Hemocyte Count levels, Glucose levels, Protein levels, Triglycerides levels. Water quality assessment was carried out to test the potentiality of bacteria Bacillus pumilus as probiotic.

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