Author(s): P. Sivakumar, M.R. Rajan

Abstract: The present study deals with the isolation, enzymatic and antibacterial activity of intestinal bacteria of Yellow molly and its role on growth. The intestinal sample was collected by dissecting the abdomen of the fish. The sample was serially diluted and the appropriate dilutions 10-6 were selected for the isolation of bacteria. The serially diluted sample was plated over sterilized nutrient agar medium and incubated at 37oC for 24hrs. The isolated and identified bacteria such as Pseudomonas sp., Streptococcus sp., Staphylococcus sp., E. coli. sp., and Aeromonas sp., were subjected for its efficacy to produce enzymes like amylase, cellulase, lipase and protease. Antibacterial activity of intestinal bacteria was also carried out. Six experimental feeds having different concentration of Pseudomonas sp., such as FI control (without probiotics), F2 (1ml), F3 (2ml), F4 (3ml), F5 (4ml) and F6 (1ml of yeast) were prepared by using fish meal, groundnut oil cake, wheat flour and tapioca. Fish were fed with the feed at a rate of 4% of live body weight and reared for a period of 45 days. Growth parameters such as condition factor, FC, FCE, FCR, Growth, PG, RGR, Assimilation, Metabolism, GGE and NGE were calculated after 45 days and ANOVA was studied for FC, Growth, GGE and NGE. Results showed that growth parameters higher in F5 containing 4ml of Pseudomonas sp., and lower in F1 control.

Pages: 330-333  |  1260 Views  65 Downloads

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