Author(s): Jag Pal, Hari Om Verma, Vijay Kumar Munka, Satyendra Kumar Maurya, Deepayan Roy, Jitendra Kumar

Abstract: Biological method of chitin extraction from crustacean shells is an advanced and new technique which involves extraction of long chain carbohydrate polymer chitin by using lactic acid bacteria and it produces a good quality end product. This eco-friendly and low cost technique requires 24-48 hours old Lactobacillus inoculums for fermentation. The 10% culture inoculums is added with crustacean shells along with same quantity of carbon sources for 180 hrs incubation followed by filtering and the obtained solid cake i.e. chitin is dried in hot air oven. The additional advantage of this technique is production of liquid fraction which is rich in protein and can be used for human and animal feed. However, this method is still limited in laboratory condition as because demineralization and deproteinization have not yet reached up to the desired yields as compared to chemical method.

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