International Journal of Fisheries and Aquatic Studies

Volume 1, Issue 5, 2014

 

Effect of humic acid on the brain tissue of brown trout treated with cadmium

 

Author(s):Ahmet Topal, Muhammed Atamanalp, Gonca Alak, Ertan Oruç, Esat Mahmut Kocaman, Yavuz Selim Sağlam

Abstract: The present study was aimed to investigate histopathological changes in brain tissue following cadmium (Cd) treatment and to observe whether humic acid (HA) has an effect against to brain damage caused by cadmium. For this aim, brown trout were randomly separated into four groups: (I) control group, (II) cadmium group (2 ppm), (III) humic acid group (5 ppm), (IV) humic acid +cadmium group. The brown trout were exposed to heavy metal stress cadmium at 2 ppm dosage and humic acid at 5 ppm dosage. After the fish were sacrificed, brain tissues were examined histopathologically by light microscopy. The histopathological results indicated damage of the fish brain tissues in the Cd and Cd+HA groups. However, HA had no effect when compared to the other groups.

Normal histologic layers of cerebellum and Purkinje cells with nuclei (arrows) in control group. H-E.

Fig: Normal histologic layers of cerebellum and Purkinje cells with nuclei (arrows) in control group. H-E.

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