Author(s): Jigarkumar Rana, Ekhalak Ansari, Dipak Patel, Minal K Kamle and Padmaja S Prabhu

Abstract: Present study was designed to evaluate the effect of 3, 4-Dichloroaniline (DCA) on the growth rate of juvenile zebrafish (Danio rerio) according to guidelines of OECD 215 and EC 14. Healthy juvenile zebrafish (1.5 month old) having individual body weight 50 -100 mg was exposed to 0.016, 0.031, 0.063, 0.125, and 0.250 mg DCA/L along with control group for 28 days. Study was performed under semi-static condition where media was renewed at every 24 hour intervals. Surviving juvenile fishes were weighted (blotted dry) replicate vise on days 14 and 28. Based on body weight average specific growth rate was calculated replicate wise. Test medium was analysed for active ingredient concentration and stability for the lowest (0.016 mg/L) and the highest (0.5 mg/L) tested concentration along with control when freshly prepared and immediately prior to renewal on days 0, 7, 14, and 21. The active ingredient concentration and stability of test item in test media was 80% of nominal concentration. All juvenile fishes were normal and no mortality was observed at the concentration levels of 0.016, 0.031, 0.063, 0.125, and 0.250 mg DCA/L and in control group. The No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC) up to 28 day exposure period of DCA were found 0.063 and 0.125 mg/L, respectively. The EC₅₀ values of DCA for average specific growth rate between days 0 and 14, 14 and 28, and 0 and 28 were 0.144, 0.178, and 0.257 mg/L, respectively. Based on the results, it can be concluded that DCA has toxic effect on growth of juvenile zebrafish. DCA affected body weight of juvenile zebrafish at the test concentration levels of 0.031, 0.063, 0.125, 0.250, and 0.500 mg DCA/L.

Pages: 456-460  |  532 Views  140 Downloads

Download Full Article: Click Here