Author(s): Rose Gregoria PJ and Dr. Moses Inbaraj R

Abstract: Sex determination and early gonadal development depends upon specific embryonic gene program that activate differentiation of the bipotential primodium. Many studies reveal that cyp19a1a and sox9a are significant for the sex determination and differentiation of ovary and testes development in vertebrates. As the research specimen Catla catla, Indian major carp is an important edible fish and has great market demand, it is imperative that the basic developmental mechanisms of sex determination are further studied in this species. It is subjected to gene expression studies in order to find out the influence of the genes cyp19a1a and sox9a in controlling the sex determination and differentiation during embryonic stages. In the present investigation cyp19a1a and sox9a are on track to express from 12^th and 30^th hpf respectively. It is seen that the expression of these genes initiated in two different times and both gene expressions were in progress throughout the study phase. This kind of expression pattern disclose the role of these genes in C. catla for sex determination and differentiation which would help to unlock the innovative fraction of research as gene manipulation programmed for increasing the mass production.

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