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International Journal of Fisheries and Aquatic Studies
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(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2019, Vol. 7, Issue 4, Part C

Bacillus tequilensis, a novel unionized ammonia-nitrogen degrading bacterium isolated from tilapia pond soil


Author(s): Reyes Alvin T, Jorduela Eric L and Doctolero Jemuel S

Abstract: The objective of this study was to isolate, characterize and identify unionized ammonia-nitrogen (NH3-N) degrading bacteria from tilapia pond soil. Eight bacterial colonies were isolated from diluted soil sample based upon colonial characteristics and growth pattern in solid and liquid medium. The isolated colonies were subjected to NH3-N degrading experiment. In all isolates used, isolate C showed the most reliable result after 72 hours incubation: 99.32%, 4.76%, 56.00%, 54.55% and 40.00% NH3-N reductions in experimental set-ups with 10, 20, 30, 40 and 50 mg/L Ammonium Chloride (NH4Cl), respectively. Morphological, biochemical and physiological tests revealed that Isolate C was Gram-positive, rod shape, positive to catalase and citric acid utilization tests, negative to urea hydrolysis and phenylalanine deamination tests, and had the ability to grow in medium supplemented with 6.5% salt. Isolate C was subjected to DNA isolation, purification and sequencing. BLAST analysis of sequences derived from both revealed that the isolate belongs as to genus Bacillus. Highest hits indicated that the species is tequilensis. This is one of the first studies that describe the ability of the bacterium to degrade or take-up NH3-N in their cells.

Pages: 161-165  |  143 Views  13 Downloads

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How to cite this article:
Reyes Alvin T, Jorduela Eric L and Doctolero Jemuel S. Bacillus tequilensis, a novel unionized ammonia-nitrogen degrading bacterium isolated from tilapia pond soil. International Journal of Fisheries and Aquatic Studies. 2019; 7(4): 161-165.
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