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International Journal of Fisheries and Aquatic Studies
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(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2019, Vol. 7, Issue 4, Part B

Differentiation of two Pangasius species, Pangasius krempfi and Pangasius mekongensis using inter-sequence simple repeat markers


Author(s): Tran Thi Minh Ly and Duong Thuy Yen

Abstract: Pangasius krempfi and Pangasius mekongensis are economically important species of catfish family Pangasiidae.Compared to P. mekongensis, P. krempfihas highervalues and has been cultured recently in the Mekong Delta, Viet Nam. Wild catfish fingerlings (<10 grams) caught in Mekong estuaries consist of both species, which are hardly distinguishableusing morphological methods. This study aimed to search for effective Inter-sequence simple repeat (ISSR) markers that can be used to quickly identify these two species. Samples were collected in different locations of the lower Mekong River. Six primers with clear and species-specific bands were selected to analyze 11 samples per species. Totally, 62 bands were detected with a size range from 500 bp to 2400 bp, in which 22 and 31private bands were found in P. krempfi and P. mekongensis, respectively. Nei’s genetic distance between the two species was 0.742 and their genetic difference GST was 0.655. Principal coordinates analysis showed that the two speciesclustered in two separate groups. The results indicate that six ISSR markers used in the present study can differentiate P. krempfi and P. mekongensis.

Pages: 116-120  |  150 Views  29 Downloads

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How to cite this article:
Tran Thi Minh Ly and Duong Thuy Yen. Differentiation of two Pangasius species, Pangasius krempfi and Pangasius mekongensis using inter-sequence simple repeat markers. International Journal of Fisheries and Aquatic Studies. 2019; 7(4): 116-120.
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