Author(s): Mushtaque Ali Jakhrani, Farkhanda Zaman Dayo, Shahid Ali Jakhrani, Wahid Bux Jatoi, Muhammad Qasim and Sahrish Memon

Abstract: The aim of present study was to compare the efficiency of two methods for analyze of essential element contents in different parts of commercially important fish species Rohu (Labeo rohita) and Morakhi (Cirrhinus mrigala) collected from Guddu Barrage Sindh. The Essential elements Sodium (Na), Potassium (K), Calcium (Ca) and Magnesium (Mg) were evaluated by using Atomic Absorption Spectrophotometer AA-100. For comparative studies different sizes of fish species were sampled during year 2016 were shifted to the laboratories for performing dissection and digestions of respective fish organs i.e. gills, liver and muscle. Both homogenous and dried methods were employed for digestion of fish samples in order to evaluate the efficiency of both methods for best mineralization. Thus findings of present study were indicated that in Rohu fish samples, concentrations of essential elements Na, K, Ca and Mg were noted in the range of (0.35-2.9, 8.4-13.7, 8.0-50.0 & 3.5-33.6 μg.g-1) and (1.0-11.76, 1.0-12.0, 0.5-9.8 & 5.3-35.4μg.g-1) for homogeneous and dried digestion methods respectively. While in case of Morakhi fish samples the concentration of respective metals were observed in range of (1.1-3.7, 0.3-13.0, 8.10-22.7 & 0.3-33.4 μg.g-1) for homogeneous digestion and (1.0-10.4, 0.30-35.7, 1.1-10.0 & 3.0-37.5 μg.g-1) for dried digestion method respectively. It has been observed that best mineralization results were achieved in the samples which were digested by Dried digestion method as compared to homogenous method due to different composition of tri-acids (HNO3, H2SO4, HClO4 in ratio of 1:4:8). While for the homogenous digestion method the composition of acids was (HNO3, HCl, H2O2 in ratio of 6:4:2).

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