Author(s): Keerthika V, R Ramesh and MR Rajan

Abstract: The present study deals with the toxicity of Iron oxide nanoparticles in Labeo rohita. The iron oxide nanoparticles were synthesized by chemical precipitation method and characterized using Scanning Electron Microscopy, Energy Dispersive X-Ray Spectroscopy and Fourier Transform Infrared spectroscopy. SEM image of iron oxide nanoparticles shows spherical shape. EDAX spectrum showed three peaks located between 2KeV and 10KeV.The FTIR spectrum of iron oxide nanoparticles was observed and spectral bands confirm the formation of Fe–O bonds. Preliminary toxicity tests were conducted for the determination of 96 h median lethal concentration (LC₅₀) of Fe₃O₄ to L. rohita in the concentration of 100ppm, 1500ppm and 3000ppm for four days and mortality observed at high concentration. The concentration equivalent to 1/10 and 1/100^th of LC₅₀ of Fe₃O₄ nanoparticles was selected for sublethal concentration of toxicological for about seven days and no mortality observed during this period. Haematological parameters and biochemical parameters such as protein, carbohydrate and lipid were estimated for control and treatments (300ppm and 30ppm) when L. rohita was exposed to Fe₃O₄ nanoparticles for a period of 7 days. The haematological parameters such as RBC, Hb and Hct were decreased and WBC increased in Fe₃O₄ nanoparticles treated fish when compared to control. Total protein, carbohydrate and lipid were consequently decreased in Fe₃O₄ nanoparticles treated fish when compared with control.

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