International Journal of Fisheries and Aquatic Studies
2017, Vol. 5, Issue 2, Part G
Mitochondrial COI gene Sequence of giant freshwater prawn, Macrobrachium rosenbergii: An assessment of a community-based stock enhancement programme in Petagas River, Sabah, Malaysia
Author(s): Mohd Samsul Rohizad Maidin, Ann Anton, Annita Seok Kian Yong and Grace Joy Wei Lie Chin
Abstract: In order to offset the decline in the population dynamics of fisheries of the Petagas River, Sabah, a community-based stock enhancement project was initiated by the Borneo Marine Research Institute (BMRI) of University Malaysia Sabah to increase the population of giant freshwater prawn,
Macrobrachium rosenbergii and its natural spawning in the wild. A total of 38,000 of hatchery reared
M. rosenbergii juveniles have been released in the Petagas River from year 2012 to 2015. In order to assess the success of stock enhancement, sequences of the mitochondrial cytochrome oxidase subunit I (
COI) gene of
M. rosenbergii reared in the BMRI hatchery and the samples caught in Petagas River were analyzed. In the present study, a total of 46 individuals (18 adults and 28 juveniles) were collected from both BMRI shrimp hatchery and Petagas River.
COI sequences of 600 to 657 bp were successfully obtained. Sequence similarity analyses showed that all samples from BMRI shrimp hatchery were identified as
M. rosenbergii (89% to 99% sequence similarity) while samples from Petagas River were identified as
M. rosenbergii (89% to 95%),
M. mammilodactylus (83% to 99%),
Macrobrachium sp. (84% to 86%),
Caridina gracilipes (91% to 92%),
Caridina sp. (81% to 87%),
Litopenaeus stylirostris (83%) and
Metapenaeus ensis (99%). The phylogenetic tree of
COI gene produced three clusters of
Macrobrachium genus, separated with its closely related species. Genetic distances between
M. rosenbergii from BMRI shrimp hatchery and
M. rosenbergii caught in Petagas River ranged from 0.00% to 13.8%. It suggested that the
M. rosenbergii released in stock enhancement programme were closely related to the
M. rosenbergii caught. Abundant individuals of
M. mammilodactylus were also caught in the river indicating that the species is the dominant species and
M. rosenbergii has yet to be established in its population following the stock enhancement practice. This molecular analysis of
Macrobrachium sp. will provide baseline information on existing species in Petagas River as well assessing the effectiveness of this stock enhancement programme.
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How to cite this article:
Mohd Samsul Rohizad Maidin, Ann Anton, Annita Seok Kian Yong, Grace Joy Wei Lie Chin. Mitochondrial COI gene Sequence of giant freshwater prawn, Macrobrachium rosenbergii: An assessment of a community-based stock enhancement programme in Petagas River, Sabah, Malaysia. Int J Fish Aquat Stud 2017;5(2):518-526.
