Contact: +91-9711224068
International Journal of Fisheries and Aquatic Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

(ICV-Poland) Impact Value: 76.37, Impact Factor: RJIF 5.69
E-ISSN: 2347-5129, P-ISSN: 2394-0506

International Journal of Fisheries and Aquatic Studies

2017, Vol. 5, Issue 2, Part A

Isolation and characterization of trypsin from fish viscera of Oil Sardine (Sardinella longiceps)


Author(s): Ajay S Khandagale, Likhitha Mundodi and Balladka Kunhanna Sarojini

Abstract: The development of the fisheries industry has led to the increased interest in the full utilization of non-edible seafood fish viscera as a large source of unique digestive enzymes especially the proteases. We report the purification and biochemical characterization of a trypsin from the viscera of Oil Sardine (Sardinella longiceps) using anion-exchange and soybean trypsin inhibitor (SBTI) affinity chromatography. Final enzyme preparation was homogeneous in SDS-PAGE and the molecular weight of the purified enzymes was estimated to be 24,000 Da. The enzyme activity was optimum at 60 °C for hydrolysis of benzoyl-dl-arginine-p-nitroanilide (BAPNA). The purified enzyme maximal activity was observed at pH 8.0. Isolated trypsin was strongly inhibited by SBTI and N-p-tosyl-1-lysine chloromethyl ketone (TLCK) which are the specific inhibitors of trypsin and was markedly inhibited by the serine-protease inhibitor PMSF. Results signify that Oil sardine viscera are useful source for production of enzymes that could be used as a biotechnological tool in various industries.

Pages: 33-37  |  568 Views  27 Downloads

Download Full Article: Click Here

How to cite this article:
Ajay S Khandagale, Likhitha Mundodi and Balladka Kunhanna Sarojini. Isolation and characterization of trypsin from fish viscera of Oil Sardine (Sardinella longiceps). International Journal of Fisheries and Aquatic Studies. 2017; 5(2): 33-37.
ADVERTISEMENT
Mosquito Journal
Call for book chapter
Journals List Click Here Research Journals Research Journals