International Journal of Fisheries and Aquatic Studies
2016, Vol. 4, Issue 3, Part F
Opportunistic pathogen Klebsiella pneumoniae isolated from Maldive’s clown fish Amphiprion nigripes with hemorrhages at Agatti Island, Lakshadweep archipelagoAuthor(s):
Mohan Gopi, Thipramalai Thankappanpillai Ajith Kumar and Sanjeevi PrakashAbstract: Objective:
To investigate the cause of mortality of Maldive’s clownfish, Amphiprion nigripes
during the culture period in ornamental fish hatchery, Agatti Island, Lakshadweep archipelago using molecular techniques.
Methods: During cultured period august 2010 the fishes were died with skin hemorrhages. Bacteria were isolated from the infected parts of liver, kidney and tissue of the fish using Nutrient agar and Zobell Marine Agar and the causative bacteria has been identified using 16S rDNA sequencing and confirmed with gene bank. The physico-chemical parameters were analyzed at the time of acclimatization and experimental period.
Results: The predominant bacterial morphology was identified as gram-negative rod-shaped, motile bacteria. Based on biochemical tests and sequence of 16S rDNA, the causative bacteria were identified as K. pneumoniae (Accession no. HQ589912). Bacterial cells were isolated from liver and kidney of all artificially infected moribund fish and confirmed as K. pneumoniae by morphological and biochemical characteristics. Fishes artificially infected by same causative agent, mortality was observed on 48th hour and no fish died within 24 hours of post exposure.
Conclusion: K. pneumoniae could be considered as an opportunistic pathogen and a symptom including hemorrhages, ulcers, and redness of the skin and it’s actively causing disease when favorable conditions occurred like increasing of un-ionized ammonia in culture tank. Pages: 464-467 | 736 Views 29 DownloadsDownload Full Article:
How to cite this article:
Mohan Gopi, Thipramalai Thankappanpillai Ajith Kumar and Sanjeevi Prakash. Opportunistic pathogen Klebsiella pneumoniae isolated from Maldive’s clown fish Amphiprion nigripes with hemorrhages at Agatti Island, Lakshadweep archipelago. International Journal of Fisheries and Aquatic Studies. 2016; 4(3): 464-467.