2016, Vol. 4 Issue 2, Part D
Effect of exogenous hormone treatments on spermatogenesis in male grey mullet out of the spawning season
Author(s): AAEl-Darawany, Amira Moustafa, Kh M Al-Marakby, AE Nasr, Mohamed AE Naiel, Yaser Hosny Ali Elewa
Abstract: The aim of the present study is to investigate the effects of human chorionic gonadotropin (hCG), carp pituitary extract (CPE), melatonin, and adrenal gland extract (AE) administered either separately or in combination on the induction of testicular maturation and spermatogenesis in the thin-lipped grey mullet, Liza ramada, out of its spawning season. The results revealed that testes obtained from fish out of their reproductive season have empty seminiferous tubules with germ cells or Sertoli cells markers barely expressed and a low gonadsomatic index (GSI). A significant increase in GSI levels was observed by different hormone treatments. As predicted, hormone treatments showed an obvious stimulation of germ cells proliferation and testicular maturation as indicated by the immunohistochemical expression of germ cells and Sertoli cells markers with the highest values achieved by hCG/AE treatment. We conclude that hormone treatments may be a potential strategy for the induction of spermatogenesis and testes maturation.
Pages: 297-302 | 643 Views 42 Downloads