Author(s): Arpita Rakshit, Aditya Paul, Somnath Bhattacharjee, Tanmoy Banik, Rita Saran, Banasree Mandal, Deep Poddar, Krishna Gangopadhyay

Abstract: Cytogenetic and genomic studies in recent years play a vital role in clarifying the considerable importance regarding species characterization, evolution and systemic studies. Spotted snakehead fish [Channa punctatus], belonging to family Channidae, collected from Nadia, Hooghly and North 24 Parganas, the three districts of West Bengal, [two sites from each district], India, and was characterized by karyotype analysis with Nucleolus Organizer Regions [NORs] and RAPD markers. Nucleolus Organizer Regions [NORs] are usually detected on the metaphase and interphase fish chromosomes through controlled silver nitrate staining technique. RAPD profiles were generated using eight oligonucleotide primers which produced repeatable and consistent results. Karyotypic study with NOR and the RAPD study of the C. punctatus species, from three districts of West Bengal, India, shows emphasis on intraspecies diversity in C. punctatus species. Therefore, the differences in results might not be only due to geographical differentiation, but also due to genetic differences of initial founder individual [s] between geographical populations which were widely migrated as a result of area effects like flood of river, founder events, genetic drift, reduced gene flow, climatic and other local adaptations, and also by transposable genetic elements.

Pages: 312-319  |  1376 Views  105 Downloads

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