Author(s): Arpita Rakshit, Krishna Gangopadhyay

Abstract: Various fish species serve as important source of protein in the diet, so it is important to assess whether fish can serve as an early warning for potential threat both for aquatic organism as well as consumer of fish. Due to various aanthropogenic activities, different hazardous chemicals as well as heavy metals reach into water bodies through run off and ultimately affects aquatic ecosystem and it has been increased immensely over the past 50 years due to industrialization and urbanization. The present study deals with the cytological effects of effluents discharged into respective sites of rural and urban importance using Channa punctatus from the water as in situ sentinels by karyotyping as well as cytochrome c oxidase gene analysis. The study also aims to gain more insight into the relationship between the chromosomal differentiation and pollution. The normal number of chromosome in control group of Channa punctatus is 2n=32, consisting of 16 metacentric, 14 submetacentric and 2 acrocentric chromosomes. Different chromosomal aberrations like End to End joining, Chromosome Break and Gap, Pycnosis, Stubbed Arm, Acentric Fragmented chromosome, Attenuated chromosomes are found in Channa species collected from two polluted sites. The difference in banding pattern was found in study of cytochrome c oxidase gene PCR products which may be due to difference in nucleotide sequences resulting from point mutation or deletion or inversion or translocation.

Pages: 233-238  |  1277 Views  67 Downloads

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